Improved silver staining of nucleolar organiser regions in paraffin wax sections using an inverted incubation technique.

Improved silver staining of nucleolar organiser regions in paraffin wax sections using an inverted incubation technique.

A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several additional advantages over other publish...

Nucleolar organiser regions in pathology.

Traditional approaches to pathological diagnosis include macroscopic examination and formalin fixation with the preparation of paraffin sections stained with a variety of histochemical techniques. These routine approaches however may be disappointing in the assessment of many conditions and occasionally fail to render a precise diagnosis or provide adequate prognostic information. Consequently ...

Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin's lymphomas using immunogold-silver staining technique.

The immunogold-silver staining (IGSS) method is a new immunostaining technique with much enhanced sensitivity for demonstration of antigens in paraffin sections. A series of 10 non-Hodgkin's lymphomas of B cell type were stained for surface membrane immunoglobulins by the IGSS and peroxidase-antiperoxidase (PAP) methods using paraffin sections and polyclonal primary antisera. The resulting stai...

Nucleolar organiser regions in renal tumours.

The mass staining of paraffin sections before the removal of wax.

The problem of producing large nwmbers of sections for teaching purposes and staining them by haematoxylin and eosin has become acute in.the post-war years, due to the shortage both of trained technicians and of reagents, especially ethyl alcohol. Methods previously used have suffered from the disadvantages of being too long, or wasteful of reagents, and it was with these factors in mind that t...